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Thread subject: compréhension des pourcentages d'identités

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compréhension des pourcentages d'identités
lea19
21 Mar 2021 17:05
Non evaluated contribution

bonjour,

Peut on continuer à travailler avec une séquence où son pourcentage d'identités est égale a 90 % ; et aussi 91%

ci joint ;

NAD(P)-dependent oxidoreductase [Pelagibacterales bacterium SAG-MED48]

Sequence ID: MBD1133843.1Length: 289Number of Matches: 1

Range 1: 1 to 219GenPeptGraphicsNext MatchPrevious Match

Alignment statistics for match #1
Score Expect Method Identities Positives Gaps Frame
349 bits(895) 3e-115 Compositional matrix adjust. 197/219(90%) 210/219(95%) 0/219(0%) -2
Query  659  MSCNVAFIGLGVMGFPMAGYISKGGHNVTVYNRTASKADKWLSEYKGQkadtpakaaaga  480
            MSCNVAFIGLGVMG+PMAGYISK GHNVTVYNRTA+KADKW++EYKG KADTPAKAA GA
Sbjct  1    MSCNVAFIGLGVMGYPMAGYISKAGHNVTVYNRTAAKADKWIAEYKGSKADTPAKAAEGA  60

Query  479  DFIFTCVGNDKDLREVTFGETGIFKTIKKGAVYIDNTTASATIAREIYDYSKKNNFGFLD  300
            DF+FTCVGND DLREVTFGE GIFKTIKKGA+YIDNTTASATI+REIYDY+KKN FG LD
Sbjct  61   DFVFTCVGNDNDLREVTFGENGIFKTIKKGAIYIDNTTASATISREIYDYAKKNEFGSLD  120

Query  299  APVSGGQAGAENGALTVMIGGDEKDFEKAKDKIDCYskkmkllgksgsgqlaKMVNQICI  120
            APVSGGQAGAENGALTVMIGGD+ DF+KAKDKIDCYSKKMKLLG +G+GQLAKMVNQICI
Sbjct  121  APVSGGQAGAENGALTVMIGGDQADFDKAKDKIDCYSKKMKLLGGAGNGQLAKMVNQICI  180

Query  119  AGLVQGLSEAINFGMKAGLNMKDVIEVISKGAAQSWQME  3
            AGLVQGLSEAINFGMKAGLNM+DVIEVISKGAAQSWQME
Sbjct  181  AGLVQGLSEAINFGMKAGLNMEDVIEVISKGAAQSWQME  219


DownloadGenPeptGraphicsNextPreviousDescriptions

NAD(P)-dependent oxidoreductase [Pelagibacterales bacterium SAG-MED49]

Sequence ID: MBD1136779.1Length: 289Number of Matches: 1

Range 1: 1 to 219GenPeptGraphicsNext MatchPrevious Match

Alignment statistics for match #1
Score Expect Method Identities Positives Gaps Frame
348 bits(894) 5e-115 Compositional matrix adjust. 196/219(89%) 210/219(95%) 0/219(0%) -2
Query  659  MSCNVAFIGLGVMGFPMAGYISKGGHNVTVYNRTASKADKWLSEYKGQkadtpakaaaga  480
            MSCNVAFIGLGVMG+PMAGYISK GHNVTVYNRTA+KADKW+++YKG KADTPAKAA GA
Sbjct  1    MSCNVAFIGLGVMGYPMAGYISKAGHNVTVYNRTATKADKWIAQYKGSKADTPAKAAEGA  60

Query  479  DFIFTCVGNDKDLREVTFGETGIFKTIKKGAVYIDNTTASATIAREIYDYSKKNNFGFLD  300
            DF+FTCVGND DLREVTFGE GIFKTIKKGA+YIDNTTASATI+REIYDY+KKN FG LD
Sbjct  61   DFVFTCVGNDNDLREVTFGENGIFKTIKKGAIYIDNTTASATISREIYDYAKKNEFGSLD  120

Query  299  APVSGGQAGAENGALTVMIGGDEKDFEKAKDKIDCYskkmkllgksgsgqlaKMVNQICI  120
            APVSGGQAGAENGALTVMIGGD+ DF+KAKDKIDCYSKKMKLLG +G+GQLAKMVNQICI
Sbjct  121  APVSGGQAGAENGALTVMIGGDQADFDKAKDKIDCYSKKMKLLGSAGNGQLAKMVNQICI  180

Query  119  AGLVQGLSEAINFGMKAGLNMKDVIEVISKGAAQSWQME  3
            AGLVQGLSEAINFGMKAGLNM+DVIEVISKGAAQSWQME
Sbjct  181  AGLVQGLSEAINFGMKAGLNMEDVIEVISKGAAQSWQME  219

DownloadGenPeptGraphicsNextPreviousDescriptions

NAD(P)-dependent oxidoreductase [Pelagibacterales bacterium]

Sequence ID: RZO61824.1Length: 289Number of Matches: 1

Range 1: 1 to 219GenPeptGraphicsNext MatchPrevious Match

Alignment statistics for match #1
Score Expect Method Identities Positives Gaps Frame
348 bits(894) 5e-115 Compositional matrix adjust. 199/219(91%) 209/219(95%) 0/219(0%) -2
Query  659  MSCNVAFIGLGVMGFPMAGYISKGGHNVTVYNRTASKADKWLSEYKGQkadtpakaaaga  480
            M+CNVAFIGLGVMG+PMAGYISK GHNVTV+NRT SKADKWLSEYKG KADTPAKAAA A
Sbjct  1    MTCNVAFIGLGVMGYPMAGYISKAGHNVTVFNRTTSKADKWLSEYKGTKADTPAKAAASA  60

Query  479  DFIFTCVGNDKDLREVTFGETGIFKTIKKGAVYIDNTTASATIAREIYDYSKKNNFGFLD  300
            D+IFTCVGND DLREVTFGE+GIFKTIKKG+VYIDNTTASATIAREIY+YSKKN FGFLD
Sbjct  61   DYIFTCVGNDNDLREVTFGESGIFKTIKKGSVYIDNTTASATIAREIYEYSKKNGFGFLD  120

Query  299  APVSGGQAGAENGALTVMIGGDEKDFEKAKDKIDCYskkmkllgksgsgqlaKMVNQICI  120
            APVSGGQAGAENGALTVMIGGD+ DF+KAKDKIDCYSKKMKLLG +GSGQLAKMVNQICI
Sbjct  121  APVSGGQAGAENGALTVMIGGDQVDFDKAKDKIDCYSKKMKLLGGAGSGQLAKMVNQICI  180

Query  119  AGLVQGLSEAINFGMKAGLNMKDVIEVISKGAAQSWQME  3
            AGLVQGLSE INFGMKAGLNM DVIEVISKGAAQSWQME
Sbjct  181  AGLVQGLSEGINFGMKAGLNMTDVIEVISKGAAQSWQME  219



 

Meglecz20CTES
21 Mar 2021 17:14
Game master

Bonjour Léa,


Si vous êtes coincée et vous ne trouvez pas meilleures séquences, OK, vous pouvez travailler avec cette séquence. Attention, si vos séquences sont trop similaires, il sera peut-être difficile à dégager un signal phylogénétique.

 

Bon travail,
Emese Meglecz