ASMA
29 Oct 2009 10:56
Contribution: Refer to Rule Book & FAQ |
suite à la réalisation du blast p nr , on a obtenu des scores entre 78 et 53 et des E values de 4 E-13 à 1 E-4.On a pas pu obtenir un bon alignement multiple avec EBI clustalW2.Parallèlement , on a effectué un blast p environnemental, on a obtenu des scores entre 431 et 50, et des values de 7E-120 à 3E-5 avec des hypothetical proteins GOS_.On suppose que l'on a affaire à une bactérie nouvelle qui présente des homologies avec des bactéries non encore annotées.Comment faire pour poursuivre notre travail merci bien. |
ETalla09
29 Oct 2009 12:47
Game master |
Bonjour,
J'ai refait le meme BlastP vs NR que vous, et j'obtiens les memes resulats que vous (ci dessous). Ce qui est important et qui doit guider votre approche scientifique est la recherche d'homologues à partir de ces alignements Blast, et non l'obtention d'un bon alignement alignement multiple. En clair, dans votre cas, les 100 premiers hits sont des homologues à votre proteine. Plusieurs evidences: Evalue de l'ordre 10e-8- 10e-10; presence d'un domaine fonctionnel de type Peptidase; pour confirmation, le hit "ref|YP_003245852.1| " presente aussi clairement ce domaine peptidase. Toutes ces proteines appartiennent clairement à cette famille de peptidase, et d'ailleurs les regions de similarités sont localises au niveau de ce domaine fonctionnel.
Resultats des courses et sur la base de ces conseils:
-Refaites votre blastp vs NR et determiner clairement vos homologues
-Refaites votre alignement multiple avec les homologues trouves
-.... en somme, Continuer normalement l'annotation de votre sequence comme prevue dans les regles du jeu
-Vous n'avez pas besoin (à cette etape de l'annotation) de penser au Blastp/x vs envir...
Bon courage,
ET
Score E
Sequences producing significant alignments: (Bits) Value
ref|ZP_05747956.1| metalloendopeptidase [Erysipelothrix rhusi... 78.2 4e-13
ref|YP_003202171.1| Peptidase M23 [Nakamurella multipartita D... 68.9 3e-10
ref|YP_001697519.1| hypothetical protein Bsph_1795 [Lysinibac... 68.6 4e-10 Gene info
ref|ZP_01171046.1| hypothetical protein B14911_10382 [Bacillu... 68.2 5e-10
ref|ZP_01748637.1| hypothetical protein SSE37_03055 [Sagittul... 67.8 7e-10
ref|YP_002430124.1| Peptidase M23 [Desulfatibacillum alkenivo... 67.8 7e-10 Gene info
ref|ZP_01722278.1| hypothetical protein BB14905_02205 [Bacill... 67.8 7e-10
ref|ZP_01724926.1| hypothetical protein BB14905_21563 [Bacill... 67.4 8e-10
ref|YP_617224.1| peptidase M23B [Sphingopyxis alaskensis RB22... 67.0 1e-09 Gene info
ref|YP_595295.1| membrane proteins related to metalloendopept... 66.6 1e-09 Gene info
ref|YP_002549355.1| membrane protein associated metalloendope... 66.6 1e-09 Gene info
ref|YP_426955.1| peptidase M23B [Rhodospirillum rubrum ATCC 1... 66.6 1e-09 Gene info
ref|ZP_01130612.1| Membrane protein related to metalloendopep... 66.6 1e-09
ref|ZP_04075936.1| hypothetical protein bthur0013_63080 [Baci... 66.6 2e-09
ref|YP_002826053.1| membrane protein associated metalloendope... 66.2 2e-09 Gene info
ref|YP_959706.1| peptidase M23B [Marinobacter aquaeolei VT8] ... 66.2 2e-09 Gene info
ref|YP_674347.1| peptidase M23B [Mesorhizobium sp. BNC1] >gb|... 66.2 2e-09 Gene info
ref|YP_002602424.1| putative metallopeptidase [Desulfobacteri... 65.9 2e-09 Gene info
ref|YP_469771.1| metalloendopeptidase protein [Rhizobium etli... 65.9 3e-09 Gene info
ref|YP_001978506.1| probable metalloendopeptidase protein [Rh... 65.1 4e-09 Gene info
ref|YP_001419076.1| peptidase M23B [Xanthobacter autotrophicu... 65.1 4e-09 Gene info
ref|ZP_03524310.1| Peptidase M23 [Rhizobium etli GR56] 65.1 5e-09
ref|ZP_05340070.1| Peptidase M23 [Sideroxydans lithotrophicus... 64.7 5e-09
ref|YP_002881299.1| Peptidase M23 [Beutenbergia cavernae DSM ... 64.7 6e-09 Gene info
ref|YP_768177.1| putative transmembrane peptidase family prot... 64.7 6e-09 Gene info
ref|ZP_01895000.1| Membrane protein [Marinobacter algicola DG... 64.7 6e-09
ref|YP_001925854.1| Peptidase M23 [Methylobacterium populi BJ... 64.7 6e-09 Gene info
ref|YP_001640443.1| peptidase M23B [Methylobacterium extorque... 64.3 7e-09 Gene info
ref|YP_824824.1| peptidase M23B [Solibacter usitatus Ellin607... 64.3 7e-09 Gene info
ref|YP_002299193.1| peptidase M23 [Rhodospirillum centenum SW... 64.3 8e-09 Gene info
ref|YP_002975935.1| Peptidase M23 [Rhizobium leguminosarum bv... 63.9 9e-09 Gene info
ref|ZP_01132686.1| putative peptidase family M23/M37 protein ... 63.9 9e-09
ref|YP_002964212.1| putative peptidase precursor [Methylobact... 63.9 9e-09 Gene info
ref|ZP_03502884.1| Peptidase M23 [Rhizobium etli Kim 5] 63.9 9e-09
ref|YP_003069242.1| putative peptidase precursor [Methylobact... 63.9 9e-09 Gene info
ref|YP_459533.1| peptidase M23/M37 [Erythrobacter litoralis H... 63.9 1e-08 Gene info
ref|YP_002421975.1| Peptidase M23 [Methylobacterium chloromet... 63.9 1e-08 Gene info
ref|YP_003250853.1| Peptidase M23 [Fibrobacter succinogenes s... 63.9 1e-08 Gene info
ref|YP_001520681.1| D-alanyl-D-alanine carboxypeptidase, puta... 63.5 1e-08 Gene info
ref|YP_002281423.1| Peptidase M23 [Rhizobium leguminosarum bv... 63.5 1e-08 Gene info
ref|ZP_04852740.1| peptidase M23B [Paenibacillus sp. oral tax... 63.2 1e-08
ref|ZP_01307935.1| Membrane protein [Oceanobacter sp. RED65] ... 63.2 1e-08
ref|ZP_03273427.1| Peptidase M23 [Arthrospira maxima CS-328] ... 63.2 1e-08
ref|YP_526329.1| Fe-S type hydro-lyases tartrate/fumarate alp... 63.2 1e-08 Gene info
ref|ZP_01201841.1| membrane peptidase, Peptidase M23 family [... 63.2 2e-08
ref|NP_970727.1| M23/M37 peptidase domain-containing protein ... 63.2 2e-08 Gene info
ref|ZP_05791184.1| membrane proteins related to metalloendope... 63.2 2e-08
ref|ZP_01016076.1| hypothetical protein PB2503_01047 [Parvula... 63.2 2e-08
ref|YP_632620.1| cell wall endopeptidase [Myxococcus xanthus ... 63.2 2e-08 Gene info
ref|YP_002133137.1| Peptidase M23 [Anaeromyxobacter sp. K] >r... 62.8 2e-08 Gene info
ref|ZP_04769720.1| Peptidase M23 [Asticcacaulis excentricus C... 62.8 2e-08
ref|ZP_04853249.1| peptidase M23B [Paenibacillus sp. oral tax... 62.8 2e-08
ref|ZP_05574573.1| N-acetylmuramoyl-L-alanine amidase [Entero... 62.8 2e-08
ref|ZP_05498168.1| Peptidase M23 [Clostridium papyrosolvens D... 62.8 2e-08
ref|YP_002603922.1| putative metalloendopeptidase; cell wall-... 62.8 2e-08 Gene info
ref|ZP_01225614.1| conserved hypothetical protein [Aurantimon... 62.8 2e-08
ref|YP_003014424.1| Peptidase M23 [Paenibacillus sp. JDR-2] >... 62.8 2e-08 Gene info
ref|NP_967392.1| cell wall-binding protein associated metallo... 62.8 2e-08 Gene info
ref|ZP_01113162.1| metalloendopeptidase-like membrane protein... 62.8 2e-08
ref|YP_001303885.1| putative peptidase [Parabacteroides dista... 62.4 3e-08 Gene info
ref|ZP_03673720.1| M23 peptidase domain protein [Borrelia bur... 62.4 3e-08
ref|ZP_03623322.1| M23 peptidase domain protein [Borrelia bur... 62.4 3e-08
ref|NP_212396.1| hypothetical protein BB0262 [Borrelia burgdo... 62.4 3e-08 Gene info
ref|ZP_03589109.1| M23 peptidase domain protein [Borrelia bur... 62.4 3e-08
ref|YP_463946.1| peptidase M23B [Anaeromyxobacter dehalogenan... 62.0 3e-08 Gene info
ref|ZP_05342226.1| peptidase, M23/M37 family [Thalassiobium s... 62.0 3e-08
ref|NP_926677.1| hypothetical protein gll3731 [Gloeobacter vi... 62.0 3e-08 Gene info
ref|ZP_01116649.1| metalloendopeptidase-like membrane protein... 62.0 3e-08
ref|ZP_03086766.1| hypothetical protein Bbur8_00680 [Borrelia... 62.0 4e-08
ref|YP_002774965.1| hypothetical protein BBR47_54840 [Breviba... 62.0 4e-08 Gene info
ref|ZP_01302865.1| Peptidase M23/M37 [Sphingomonas sp. SKA58]... 62.0 4e-08
ref|YP_663068.1| peptidase M23B [Pseudoalteromonas atlantica ... 62.0 4e-08 Gene info
ref|YP_001377974.1| peptidase M23B [Anaeromyxobacter sp. Fw10... 62.0 4e-08 Gene info
ref|ZP_03772946.1| M23 peptidase domain protein [Borrelia sp.... 61.6 4e-08
ref|ZP_02163984.1| M23/M37 peptidase domain protein [Kordia a... 61.6 4e-08
ref|ZP_01736220.1| Membrane protein [Marinobacter sp. ELB17] ... 61.6 4e-08
ref|NP_218489.1| hypothetical protein TP0049 [Treponema palli... 61.6 4e-08 Gene info
ref|ZP_05632924.1| hypothetical protein FulcA4_05780 [Fusobac... 61.6 5e-08
ref|ZP_03436613.1| M23 peptidase domain protein [Borrelia bur... 61.6 5e-08
ref|XP_001786775.1| predicted protein [Physcomitrella patens ... 61.6 5e-08 UniGene infoGene info
ref|YP_001521310.1| M23 peptidase domain-containing protein [... 61.6 5e-08 Gene info
ref|ZP_06006147.1| YD repeat-containing protein [Prevotella b... 61.2 6e-08
ref|YP_520433.1| hypothetical protein DSY4200 [Desulfitobacte... 61.2 6e-08 Gene info
ref|ZP_01168032.1| peptidase, M23/M37 family protein [Oceanos... 61.2 6e-08
ref|ZP_05622729.1| M23/M37 peptidase domain protein [Treponem... 61.2 6e-08
ref|ZP_05103905.1| M23 peptidase domain protein [Methylophaga... 61.2 6e-08
ref|YP_003245852.1| Peptidase M23 [Geobacillus sp. Y412MC10] ... 61.2 6e-08 Gene info
ref|ZP_02037571.1| hypothetical protein BACCAP_03188 [Bactero... 61.2 7e-08
ref|ZP_05081382.1| peptidase M23B [beta proteobacterium KB13]... 61.2 7e-08
ref|YP_002457633.1| Peptidase M23 [Desulfitobacterium hafnien... 61.2 7e-08 Gene info
ref|YP_002250823.1| peptidase M23/M37 [Dictyoglomus thermophi... 61.2 7e-08 Gene info
ref|YP_001638023.1| peptidase M23B [Methylobacterium extorque... 60.8 7e-08 Gene info
ref|YP_001530365.1| peptidase M23B [Desulfococcus oleovorans ... 60.8 8e-08 Gene info
ref|ZP_04687513.1| peptidase [Streptomyces ghanaensis ATCC 14... 60.8 8e-08
ref|ZP_02037845.1| hypothetical protein BACCAP_03464 [Bactero... 60.8 8e-08
ref|ZP_01863621.1| Peptidase M23/M37 [Erythrobacter sp. SD-21... 60.8 8e-08
ref|YP_002311471.1| Peptidase M23B [Shewanella piezotolerans ... 60.8 8e-08 Gene info
ref|YP_001838037.1| M23 family metalloendopeptidase [Leptospi... 60.8 8e-08 Gene info
ref|YP_003074419.1| M23 peptidase domain protein [Teredinibac... 60.8 9e-08 Gene info
ref|ZP_01133451.1| M23/M37 peptidase domain protein [Pseudoal... 60.8 9e-08
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