Bonjour Mme,

Depuis hier je suis bloqué sur le site http://www.phylogeny.fr ; Lorsque je rentre mes séquences le message d'erreur suivant apparait:

" Error: MUSCLE input: Invalid alignment format: dataset does not appear to be an alignment! (check sequence #3) "

Je ne comprend pas , j'ai réessayé plusieurs fois , revus les vidéos et je ne comprend pas l'erreur... 

Ci-joint mes séquences, 

Bonjour,

Dans les fichiers fasta il y a une seule ligne avant la séquence avec les identifiants et les infos diverses. Vous l’avez coupée en deux par hasard cette ligne de la séquence trois.

Ça devrait être comme ceci :

>BPACCCC1   E-value=3e-169   Score=479  Bacteria;Proteobacteria;Alphaproteobacteria;Candidatus Pelagibacterales;Candidatus Pelagibacteraceae;Candidatus Pelagibacter;Candidatus Pelagibacter bacterium; WP_271564071.1 ParA family protein [Candidatus Pelagibacter bacterium]
MQIISVINQKGGVGKTTTVINLAAGLAQQDKKILVIDLDPQGNATTGLGLSNLESSTETIYGVLNGTKSI
SEVIKKTEFKNLDIVTSNVDLSGLEVETADDNMRAFILKRELTAYLNDSGATYDYVLIDCPPSLSLLTVM
ALVSSHSLLVPLQTEFFALEGLTQLMKTIERIKANLNPELKIRGILLTMFDKRNKLSSQVEKEARDYFNE
KVYLTVIPRNVRLSEAPSHGMPVLMYDKSCPGSKSYFNFTDEFINQEQKIGSAA
 
Bon travail,
Emese Meglecz

J'ai essaye et ca ne marche toujours pas ...

J\'ai essaye et ca ne marche toujours pas ...

J'ai essaye et ca ne marche toujours pas ...

Je viens de lancer l’alignement avec la ligne de séquence 3 corrigée. Ça marche. 

Je copie-colle ici le JDD corrigé. Essayez avec cela.

Courage,
Emese

>OMRGCv2_11491060 Traduction [877-1659 sens direct]
MQIISIINQKGGVGKTTTVINLAAGLAQQGKKVLIIDLDPQGNATTGLGLSNLENSSNTIYGILNGTKSIMEVIKKTEFENLDIITSNVDLSGLELETAS
DNERAFILKVKLASYLNDSSRYYDYILIDCPPSLSLLTVMALVSSHSLVVPLQTEFFALEGLTQLMKTIERIKVNLNPELKIRGILLTMYDKRNKLSSQV
EKEARDYFKEKVYLTIIPRNVRLSEAPSHGMPVLIYDKSCLGSKSYFSFTDEFINQEQKIG
 
>BPACCC1   E-value=4e-170   Score=481  Bacteria;Proteobacteria;Alphaproteobacteria;Candidatus Pelagibacterales;Candidatus Pelagibacteraceae;Candidatus Pelagibacter; WP_169035784.1 AAA family ATPase [Candidatus Pelagibacter ubique]
MQIISIINQKGGVGKTTTVINLAAGLAQQQKKILVIDLDPQGNATTGLGLSNMQNSSDTIYGVLNGTKSI
SDVIKKTKVENLDLISSNVDLSGLEVETAGDNERAFLLKVKLTAYLNDSEGFYDYILIDCPPSLSLLTVM
ALVSSNSLVVPLQAEFFALEGVTQLLKTIDRIKINLNPELKIRGILLTMYDKRNKLSSQVEKEARDYFKE
KVYITIIPRNVRLSEAPSHGMPVLIYDKSCPGSKSYFSFTDEFINQEQKIGSAA
 
>BPACCCC1   E-value=3e-169   Score=479  Bacteria;Proteobacteria;Alphaproteobacteria;Candidatus Pelagibacterales;Candidatus Pelagibacteraceae;Candidatus Pelagibacter;Candidatus Pelagibacter bacterium; WP_271564071.1 ParA family protein [Candidatus Pelagibacter bacterium]
MQIISVINQKGGVGKTTTVINLAAGLAQQDKKILVIDLDPQGNATTGLGLSNLESSTETIYGVLNGTKSI
SEVIKKTEFKNLDIVTSNVDLSGLEVETADDNMRAFILKRELTAYLNDSGATYDYVLIDCPPSLSLLTVM
ALVSSHSLLVPLQTEFFALEGLTQLMKTIERIKANLNPELKIRGILLTMFDKRNKLSSQVEKEARDYFNE
KVYLTVIPRNVRLSEAPSHGMPVLMYDKSCPGSKSYFNFTDEFINQEQKIGSAA
 
>BPACCC2   E-value=4e-161   Score=458  Bacteria;Proteobacteria;Alphaproteobacteria;Candidatus Pelagibacterales;Candidatus Pelagibacteraceae;Candidatus Pelagibacter; WP_145596565.1 ParA family protein [Candidatus Pelagibacter sp. FZCC0015]
MQIISVINQKGGVGKTTTVINLAAGLSQLNKKILVIDLDPQGNATTGLGLSNTDNSSDTIYGVLNGTKEI
YQVIKKTQFENLDLITSNVDLSGLEVETANDTNRAFILKHKLAAYLNNYRGAYDYILIDCPPSLSLLTVM
ALVCSGSLLVPLQTEFFALEGLTQLMKTIERIKVSLNPELKIRGILLTMYDKRNKLSSQVEKEARDYFSE
KVYSTVIPRNVRLSEAPSHGMPVLIYDKSCPGSKSYFSFTDEFINQERTIGSAA
 
>BPACCC3   E-value=1e-160   Score=457  Bacteria;Proteobacteria;Alphaproteobacteria;Candidatus Pelagibacterales;Candidatus Pelagibacteraceae;Candidatus Pelagibacter; WP_008544558.1 ParA family protein [Candidatus Pelagibacter sp. HTCC7211]
MQIISVINQKGGVGKTTTVINLAAGLTQLNKKILIIDLDPQGNATTGLGLSNVDNSSDTIYGVLNGTRQI
NEIIKRTQFENLDIITSNVDLSGLEVETADDSNRAFILKAKLTSYLNNSRGLYDYVLIDCPPSLSLLTVM
ALVCSNSLLVPLQTEFFALEGLTQLMKTIERIKVSLNPDLKIRGILLTMYDKRNKLSSQVEKEARDYFNE
KVYSTVIPRNVRLSEAPSHGVPVLIYDKSCPGSKSYFNFTDEFINQESTIGSAA
 
>BPACCC4   E-value=5e-105   Score=316  Bacteria;Proteobacteria;Alphaproteobacteria;Candidatus Pelagibacterales;Candidatus Pelagibacterales incertae sedis;Candidatus Fonsibacter; WP_099340930.1 AAA family ATPase [Candidatus Fonsibacter ubiquis]
MPRIISVINQKGGVGKTTTAINLAAGLALKNFKVLLIDLDPQGNASTGVGITQSEREKSIYDLLIKKDSF
AECIKKTKIENLSIIPANAELSGLETEVANNSQKAFLLKNIIDGSENILEKEKFSYIIIDCPPSLSLLTI
MSLVISHSLVVPLQAEFFALEGLSQLVKTIDRIKINLNPNLEIQGIVLTMYDKRNKLCADVEKEGRNFFG
NKVYSVNIPRNVRISEAPSFGMPVILYDKYCAGSLAYNQFVEEFLVRENLQEKKVA
 
>BPAMRH1   E-value=2e-86   Score=269  Bacteria;Proteobacteria;Alphaproteobacteria;Maricaulales;Robiginitomaculaceae;Hellea; WP_026942857.1 ParA family protein [Hellea balneolensis]
MTKPRIIAIVNQKGGVGKTTTTINLAAALTLRGKKVLLIDLDPQGNAATGLGIPRTARDVTVYDVLVESR
PISDAIMDTVVPGLKLVPSHIDLASAELEIGDRAGRTTILRHALSRVDFDYVLIDCPPSLNLLTVNALSA
ARSVLVPLQCEFFALEGLSQLLQTVEMAKANINSDLVIDGVMLTMYDQRNRLSAQVAADVRKHLGRAVFK
TVIPRNVRIAEAPSFGKPVIMYDPDCAGSKAYIDLADEMLGRHSKSRK
 
>BPAMME1   E-value=1e-90   Score=280  Bacteria;Proteobacteria;Alphaproteobacteria;Maricaulales;Maricaulaceae;Euryhalocaulis; WP_022696858.1 MULTISPECIES: AAA family ATPase [Euryhalocaulis]
MSDGTKVIAIANQKGGVGKTTTAINLGTALAASGRKVLLIDLDPQGNASTGLGLEKAKRKITAYDVVVSE
YDLEKAIQPSSIPDLDLLPSDVELSGAELELASTPRRSYRLKHAIDRRDRMVAREEAERYDFILIDCPPS
LNLLTVNAMTAADSVLVPLQCEFFALEGLTQLMRTIELVRSSLNPDLEIEGVVLTMHDRRNRLSDQVADD
VRDYFGEKVFETVIPRNVRVSEAPSFGKPAIVYDYECAGSRAYLRMARELLRRQPSEAEAA
 
>BPARRR1   E-value=2e-94   Score=289  Bacteria;Proteobacteria;Alphaproteobacteria;Rhodospirillales;Rhodospirillaceae;Rhodospirillum; WP_011391374.1 ParA family protein [Rhodospirillum rubrum]
MPDQAIAAPALSAKLVLNTPRIIAIANQKGGVGKTTTAINLATALAATGKRVLIIDMDPQGNASTGLGLS
SAARKVTTYDILMGDAKARAAVTPTGIPRLAVIPAGVDLAGAELELVERTQREFRLRMALADELIDFDYV
LVDCPPALGLLTLNALIAADAVMVPLQCEFFALEGVSHLVKTIDRVRKAFNPRLEIQGIVLTMFDRRNNL
SEMVAADVRDYFGAKVYKTVIPRNVRISEAPSHGKPVLLYDLKCAGSQAYLHLAGEIIKQEGGLAA
 
>BPARTTT1   E-value=3e-94   Score=289  Bacteria;Proteobacteria;Alphaproteobacteria;Rhodospirillales;Terasakiellaceae;Terasakiella;Terasakiella sp. A23; WP_317683005.1 ParA family protein [Terasakiella sp. A23]
MTDQTTVEVTPRIIAIANQKGGVGKTTTTINLATALAAVGKKTLVLDLDPQGNASTGLGIDHDMRSRNAY
HVMMGDIEADGAIQKSNIPNLSVIPSGPDLAGAEIEIPELDRPQFCLRESLSTIKGYDYILIDCPPALGM
LTINALVGSHAVMVPLQCEFFALEGITALVKTIERVKKNFNPSLDIQGIVLTMFDKRNNLSDMVADDVRE
FFGEKVYETVIPRNVRVSEAPSHGKPVLLYDHKCAGSKAYLHLASEVLRRENRKAA
 
>BPARAS1   E-value=8e-91   Score=280  Bacteria;Proteobacteria;Alphaproteobacteria;Rhodospirillales;Azospirillaceae;Skermanella; WP_084720641.1 AAA family ATPase [Skermanella aerolata]
MSRARVISVANQKGGVGKTTTTVNLATALAAVGKRVLIMDMDPQGNASTGLGIDRSHRSVGTYDVLFAEV
RVENAAIETPVPNLWVVTSSVDLSGAEIELVSTEQREYRLRMALAHSALNFDYVLIDCPPALGLLTLNAL
VASDAVMVPLQCEFYALEGLSHLIRTIERVKRAFNPGLDIHGVVLTMFDKRNNLSDMVAADVRGFFGEKV
YDTVIPRNVKVSEAPSHGKPVLIYDLRCPGSQAYIHLASEVLKREKKRLIA
 
>BPARTV1   E-value=4e-89   Score=276  Bacteria;Proteobacteria;Alphaproteobacteria;Rhodospirillales;Thalassospiraceae;Varunaivibrio; WP_132937597.1 ParA family protein [Varunaivibrio sulfuroxidans]
MEKKGPLEREHPRIISIANQKGGVGKTTTAINLATALAAVKQRVLLIDLDPQGNASTGLGLDQASRRRDS
YQVIIGDIALSEAIQKTAIPGLDIIPAGVDLSAAEIELVDVERREYCLRNAIGDGAAHYDYILIDCPPAL
GLLTLNALVASQAILVPLQCEFFALEGISHLMRTVAQVQKIFNHGLELQGIVLTMFDKRNNLSEQVAADV
REYFGDKVYETVIPRNVRVSEAPSYGRPVLMYDMACAGSQAYLHLAGEILRREGHKAV
 
>BPARASS1   E-value=3e-86   Score=268  Bacteria;Proteobacteria;Alphaproteobacteria;Rhodospirillales;Acetobacteraceae;Siccirubricoccus;Siccirubricoccus soli; WP_252951973.1 ParA family protein [Siccirubricoccus soli]
MSPPPSERAKPRVIALANQKGGVGKTTTAINLATVLAADRRVLLLDLDPQGNASTGLGVDRAARGAGTYA
LLTGDRPLAELIRPTPVPTLSLIPADTDLAGAEVELVALEDRDRRLRNVFTADAARLAEYDYIFLDCPPS
LGLLTLNALVAADSVLVPLQTEFFALEGVSQIVQTIERVRRALNPGLALEGIALTMYDRRNNLSELVAQD
VRGFFKEKVFETVIPRNVRVSEAPSHGLPVTLYDSRSPGAQAYVALADELRRREKVRAA
 
>BPASSS1   E-value=4e-96   Score=293  Bacteria;Proteobacteria;Alphaproteobacteria;Sneathiellales;Sneathiellaceae;Sneathiella; WP_161340534.1 AAA family ATPase [Sneathiella chungangensis]
MSNSGAGLHNPASARILAIANQKGGVGKTTTTINLATALAAIGQKVLIIDIDPQGNASTGFGIGRDDREK
NVYGVLMGETTIHAAIHKTNIPQLDLVPSTADLSGAELELIELPRRSHRLYDAVSEVRADYDYILVDCPP
SLNLLTLNAMVAVHAVIVPLQCEFFALEGLSMLIKTIERVRLTLNPGLEIQGVVLTMFDKRNNLSLQVAQ
DVRDFLGEKVYRTVIPRNVRISEAPSHGKPVLIYDHRCAGSQAYIRLASEVIRRERHLNAA
 
>BPAHRRN1   E-value=5e-90   Score=278  Bacteria;Proteobacteria;Alphaproteobacteria;Hyphomicrobiales;Rhizobiaceae;Rhizobium/Agrobacterium group;Neorhizobium; WP_105372198.1 MULTISPECIES: ParA family protein [Neorhizobium]
MFEKNRIITIANQKGGVGKTTTAINLATALAAIGERVLIVDLDPQGNASTGLGIERRERKISSYDLLMGY
NSVAETALETAVPNLWIVPSTMDLLGFEMEISQSSDRAFKLRRALNTDEAQFYSYILLDCPPSFNLLTMN
AMAAAHSVLVPLQCEFFALEGLSQLLETVDQVRRTINPALDIQGIVLTMFDARNNLAQQVVSDVRTYLGD
KVYHTLIPRNVRVSEAPSYGKPAILYDLKCAGSQAYLQLASEVIQRERRRKAA
 
>BPAHAA1   E-value=6e-93   Score=286  Bacteria;Proteobacteria;Alphaproteobacteria;Hyphomicrobiales;Afifellaceae;Afifella; WP_255599971.1 ParA family protein [Afifella sp. IM 167]
MPQNPERLPPIPRVLVIANQKGGVGKTTTAINLGTALAAIGERVLIVDLDPQGNASTGLGIGRENRLHST
YDILMDGMAIRQVARDTVVPRLSIAPSTLDLLGVELEIASLQDRAFRLRKAVADLGTEASPPYTYVLIDC
PPSLNLLTINAMAAGHAVLVPLQCEFFALEGLSQILGTVERVRRGLNPELSIHGIVLTMYDGRNNLSGQV
VEDVRSHMGEKVYDTIIPRNVRISEAPSHGKPAILYDINCAGSQAYLKLASEVIQRERVLRAA
 
>BPAHBB1   E-value=4e-93   Score=286  Bacteria;Proteobacteria;Alphaproteobacteria;Hyphomicrobiales;Breoghaniaceae;Breoghania; WP_107988727.1 ParA family protein [Breoghania corrubedonensis]
MSVLPKSPRVLSLANQKGGVGKTTTAINLGTALAAIGERVLVIDLDPQGNASTGLGIERSQRTVSTYEVL
YGEASLKDAIRETAVPRLWAAPSTMDLLGLELEIADSPDRAFRLRKAIADYVAEHDQSDFSYILVDCPPS
LNLLTINALSASHSILVPLQCEFFALEGLSQLLSTVEQVKQALNPELSIHGIVLTMYDSRNNLSTQVVAD
VRETMGEAVYDTVIPRNVRVSEAPSYGKPVLLYDLKCPGSQAYLRLASEIIQRERKIRKAAA
 
>BPAHKP1   E-value=6e-91   Score=281  Bacteria;Proteobacteria;Alphaproteobacteria;Hyphomicrobiales;Kaistiaceae;Prosthecomicrobium; WP_281380209.1 ParA family protein [Prosthecomicrobium pneumaticum]
MTQIETAALPSPAASRLPRLPRVLALANQKGGVGKTTTAINLGTALAAIGEEVLIVDLDPQGNASTGLGI
DRRSRQVSTYDLLIGEARLAEVVKETAVPRLSVAPSTLDLLGVELEIAAQSDRAFRLRRAIADHMARAES
IRGRNYTYVLVDCPPSLNLLTINALSAAHSILVPLQCEFFALEGLSQLLQTVEQVRRALNPELSIHGIVL
TMFDGRNNLSSEVVAEVRANMGDAVYDTIIPRNVRISEAPSHGKPVLLYDFKSTGSQAYLRLASEIIQRE
KRLRAA

Merci beaucoup cela fonctionne !

(Je viens de me rendre compte que mon message à été envoyé 3 fois par erreur ... désolé! )