Forum "ORF finding"

Thread subject: ORF

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ORF
Rabail
28 Apr 2011 14:34
Non evaluated contribution
>ORF number 1 in reading frame 2 on the direct strand extends from base 293 to base 868.
ATGCTTAATATAGATTTTCATGAAACTATTGGGCAAAGTCAATTTTCAGAAAAAGGAAAA
CAAATAGTTATTAATGCATTAGAAAGACAAATAGCTAAAAGAGAAGATGATGGAGCTGTT
TGTGTTTTATTAAATAGTAAGAAAAAGTATATTTTAAGAAGCAATCAAACAGCATCTTAT
ATTACTCAGGATTTAGGAGCAGCAGTTGAAAGATTTAAAAAACATAATTTTAATAAAATG
ATTTATATAACTGACTTTAGACAAAAACAGCATTTTGAAAGTTTATCTTTATTATTAAAA
GCAATGAAATATGATTTTGCAGATAATCTTATACATTTACCTTTTGGAACTGTAAAATTT
GGAAAAGAAATCATGGCTACAAGAAAAGGACAAATAATTCTTTTAAAAGATGTTTTAAAT
AAAACAATTGAAAAAGCAAAAAACAATATAAAAAAGAGAAAAACAAAAGGAAGTCCTGAA
AAAGTCGGAGTAGGAGCAATAAAATACATTATTTTAAAAAATGAACCTAAAAAAGATATT
CAATTTTCATGGGAATCAGCATTATCTTTTGAAGGA

// The orf found over NCBI had a length of 822 bases whereas there was only one orf found over sequence manipulation suite with a length of 575 nucleotides in reading frame +2 with a start codon taken as ATG.The posted orf represents that of SMS orf finder.
// we'd take our result as a true positive since it fulfills the limit of at least 150 codons.Total codons being 191.