Forum "ORF finding"

Thread subject: ORF

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ORF
ftaq
27 Apr 2011 9:51
Non evaluated contribution
>ORF number 1 in reading frame 3 on the direct strand extends from base 12 to base 431.
ATGCGCACTGTCTGCGGCAGACTGGAAAGTCGTTACAGGTATTCAGCCAGCATCGTCTAC
AACAACTTCCCTTGGCCTGCGGTGGCTACCGATGCGCTCAAGGGCACGCTGGCGAACAAA
CTGCACACCGCTATCGAGCATGCGGCGCAAGCCGTGCTCGACGCCCGCGCCTTGTTTCAA
CAAGGCGAGCACCCCGCCACACTCGCCGATTTATACGACCCGACCAGCATGCCCGCGCCC
TTGCTGCGCGCGCACCAGTTGCTAGATGCGGCCGTGGATAAAGCCTATGCCCAAACCTGC
GGCGGCGCGGCCAGTTACAAAACCGATGCCGACCGCGTGGCGTTTTTGTTCATGCTCTAC
CAGCGTTACACCAGTTTGCTGAGCGCGGATGGTGCTAAGGCCAGGCGCAGTCTCAAATAA


>Translation of ORF number 1 in reading frame 3 on the direct strand.
MRTVCGRLESRYRYSASIVYNNFPWPAVATDALKGTLANKLHTAIEHAAQAVLDARALFQ
QGEHPATLADLYDPTSMPAPLLRAHQLLDAAVDKAYAQTCGGAASYKTDADRVAFLFMLY
QRYTSLLSADGAKARRSLK*

Comments:

Found on direct strand, reading frame 3. The length of this ORF is 420 nucleotides. We selected this ORF since it is greater than 60 codons, a criteria for being a true positve hit.