NAME
23 Oct 2009 14:11
Contribution non évaluée |
Est ce que cette Evalue est suffisante pour parler d'homologie étant donné les résultats obtenus suite à un BLAST X:
Score E
Sequences producing significant alignments: (Bits) Value N
gb|CP001737.1| Nakamurella multipartita DSM 44233, complete g... 39.1 8e-08 1
emb|FM209186.1| Pseudomonas aeruginosa LESB58 complete genome... 46.4 8e-08 1
gb|CP001630.1| Actinosynnema mirum DSM 43827, complete genome 45.5 1e-07 1
gb|CP000769.1| Anaeromyxobacter sp. Fw109-5, complete genome 40.0 1e-07 1
dbj|AP009152.1| Kocuria rhizophila DC2201 DNA, complete genome 36.3 2e-06 1
emb|AM279695.1| Streptomyces ambofaciens DSM 40697 right term... 44.6 3e-06 1
emb|AM279694.1| Streptomyces ambofaciens DSM 40697 left termi... 44.6 3e-06 1
dbj|AP006618.1| Nocardia farcinica IFM 10152 DNA, complete ge... 42.8 3e-06 1
dbj|AP009493.1| Streptomyces griseus subsp. griseus NBRC 1335... 41.4 4e-06 1
gb|CP001349.1| Methylobacterium nodulans ORS 2060, complete g... 39.6 4e-06 1
gb|AE017221.1| Thermus thermophilus HB27, complete genome 45.1 4e-06 1
gb|CP001339.1| Thioalkalivibrio sp. HL-EbGR7, complete genome 51.9 5e-06 1
gb|CP000927.1| Caulobacter sp. K31, complete genome 40.5 7e-06 1
gb|CP001029.1| Methylobacterium populi BJ001, complete genome 41.8 8e-06 1
emb|CT573213.2| Frankia alni str. ACN14A chromosome, complete... 47.8 9e-06 1
gb|CP000613.1| Rhodospirillum centenum SW, complete genome 38.2 9e-06 1
gb|CP000509.1| Nocardioides sp. JS614, complete genome 43.2 1e-05 1
gb|CP000884.1| Delftia acidovorans SPH-1, complete genome 38.6 1e-05 1
gb|CP000850.1| Salinispora arenicola CNS-205, complete genome 42.3 1e-05 1
gb|CP001628.1| Micrococcus luteus NCTC 2665, complete genome 40.5 2e-05 1
gb|CP000943.1| Methylobacterium sp. 4-46, complete genome 40.9 2e-05 1
emb|AL939126.1| Streptomyces coelicolor A3(2) complete genome... 35.4 2e-05 1
gb|CP001643.1| Brachybacterium faecium DSM 4810 strain 6-10, ... 37.3 2e-05 1
gb|CP000667.1| Salinispora tropica CNB-440, complete genome 39.6 2e-05 1
gb|CP001131.1| Anaeromyxobacter sp. K, complete genome 45.5 2e-05 1
emb|AL939117.1| Streptomyces coelicolor A3(2) complete genome... 32.7 3e-05 1
gb|CP001281.2| Thauera sp. MZ1T, complete genome 39.1 4e-05 1
gb|CP000747.1| Phenylobacterium zucineum HLK1, complete genome 46.0 4e-05 1
gb|CP001700.1| Catenulispora acidiphila DSM 44928, complete g... 41.4 5e-05 1
dbj|BA000030.3| Streptomyces avermitilis MA-4680 DNA, complet... 43.2 6e-05 1
emb|BX640416.1| Bordetella pertussis strain Tohama I, complet... 46.0 6e-05 1
gb|CP000386.1| Rubrobacter xylanophilus DSM 9941, complete ge... 37.7 7e-05 1
dbj|AP008226.1| Thermus thermophilus HB8 genomic DNA, complet... 40.5 7e-05 1
dbj|AP006840.1| Symbiobacterium thermophilum IAM 14863 DNA, c... 39.6 7e-05 1
emb|CU695238.1| Ralstonia solanacearum strain MolK2 Genome Draft 35.4 8e-05 1
gb|AE004091.2| Pseudomonas aeruginosa PAO1, complete genome 38.6 1e-04 1
gb|CP001001.1| Methylobacterium radiotolerans JCM 2831, compl... 38.2 1e-04 1
gb|AF533768.1| Cercopithecine herpesvirus 1 strain E2490, com... 33.1 1e-04 1
gb|CP000438.1| Pseudomonas aeruginosa UCBPP-PA14, complete ge... 48.7 1e-04 1
gb|DQ272520.1| Streptomyces aculeolatus A gene locus, complet... 31.8 1e-04 1
emb|AL939108.1| Streptomyces coelicolor A3(2) complete genome... 40.5 1e-04 1
dbj|AP011115.1| Rhodococcus opacus B4 DNA, complete genome 41.8 2e-04 1
emb|AM238664.1| Streptomyces ambofaciens ATCC 23877 right chr... 40.9 2e-04 1
emb|AM920689.1| Xanthomonas campestris pv. campestris complet... 36.3 2e-04 1
emb|BX640447.1| Bordetella bronchiseptica strain RB50, comple... 47.3 2e-04 1
gb|CP000251.1| Anaeromyxobacter dehalogenans 2CP-C, complete ... 37.7 2e-04 1
gb|CP000249.1| Frankia sp. CcI3, complete genome 42.3 2e-04 1
gb|EU301739.1| Actinomadura kijaniata fructokinase gene, part... 37.7 3e-04 1
emb|FN554889.1| Streptomyces scabiei 87.22 complete genome 40.0 3e-04 1
gb|CP001618.1| Beutenbergia cavernae DSM 12333, complete genome 40.5 3e-04 1
gb|CP001510.1| Methylobacterium extorquens AM1, complete genome 38.2 4e-04 1
gb|CP001150.1| Rhodobacter sphaeroides KD131 chromosome 1, co... 40.5 4e-04 1
gb|CP000143.1| Rhodobacter sphaeroides 2.4.1 chromosome 1, co... 40.0 4e-04 1
dbj|BA000040.2| Bradyrhizobium japonicum USDA 110 DNA, comple... 40.9 4e-04 1
gb|CP000750.2| Kineococcus radiotolerans SRS30216, complete g... 40.5 4e-04 1
emb|FP103042.2| Methylobacterium extorquens DM4 str. DM4 chro... 40.9 5e-04 1
gb|CP000512.1| Acidovorax citrulli AAC00-1, complete genome 38.6 5e-04 1
gb|EU827593.1| Actinosynnema pretiosum subsp. pretiosum strai... 35.0 5e-04 1
gb|AF119361.1|AF119361 Frankia sp. EuIK1 NifK (nifK), NifE (n... 40.0 5e-04 1
gb|CP001359.1| Anaeromyxobacter dehalogenans 2CP-1, complete ... 42.3 5e-04 1
emb|BX640440.1| Bordetella bronchiseptica strain RB50, comple... 35.0 5e-04 1
gb|CP000820.1| Frankia sp. EAN1pec, complete genome 37.7 5e-04 1
gb|CP000699.1| Sphingomonas wittichii RW1, complete genome 46.9 6e-04 1
emb|AJ937741.1| Streptomyces ambofaciens ATCC 23877 right ter... 40.9 6e-04 1
emb|AJ937740.1| Streptomyces ambofaciens ATCC 23877 left term... 40.9 8e-04 1
emb|AL939119.1| Streptomyces coelicolor A3(2) complete genome... 35.0 8e-04 1
gb|CP000159.1| Salinibacter ruber DSM 13855, complete genome 39.6 9e-04 1
gb|CP000680.1| Pseudomonas mendocina ymp, complete genome 41.4 9e-04 1
gb|CP000751.1| Kineococcus radiotolerans SRS30216 plasmid pKR... 34.5 0.001 1
emb|AJ629247.1| Streptomyces fradiae neomycin biosynthesis ge... 36.3 0.001 1
gb|CP000113.1| Myxococcus xanthus DK 1622, complete genome 40.0 0.001 1
gb|CP000453.1| Alkalilimnicola ehrlichii MLHE-1, complete genome 40.5 0.001 1
gb|AE016958.1| Mycobacterium avium subsp. paratuberculosis st... 38.2 0.001 1
gb|CP000230.1| Rhodospirillum rubrum ATCC 11170, complete genome 38.2 0.001 1
gb|CP000529.1| Polaromonas naphthalenivorans CJ2, complete ge... 39.1 0.001 1
gb|DQ403252.1| Streptomyces fungicidicus DXP synthase 2 gene,... 39.1 0.001 1
gb|CP000928.1| Caulobacter sp. K31 plasmid pCAUL01, complete ... 36.8 0.001 1
gb|CP000577.1| Rhodobacter sphaeroides ATCC 17029 chromosome ... 40.5 0.001 1
gb|AF497482.1| Micromonospora echinospora calicheamicin biosy... 39.1 0.002 1
emb|BX640430.1| Bordetella parapertussis strain 12822, comple... 40.0 0.002 1
gb|AY498874.1| Streptomyces lavendulae linear plasmid pSLV45 ... 30.4 0.002 1
gb|DQ013294.1| Polyangium cellulosum strain So ce12 disorazol... 34.1 0.002 1
gb|CP000050.1| Xanthomonas campestris pv. campestris str. 800... 37.7 0.002 1
gb|AE017282.2| Methylococcus capsulatus str. Bath, complete g... 40.5 0.002 1
emb|AJ874112.1| Polyangium cellulosum disA gene, disB gene, d... 34.1 0.002 1
emb|BX640446.1| Bordetella bronchiseptica strain RB50, comple... 46.9 0.002 1
gb|AF509565.1| Streptomyces sp. JP95 griseorhodin biosynthesi... 37.3 0.002 1
emb|AL939121.1| Streptomyces coelicolor A3(2) complete genome... 33.6 0.002 1
gb|CP000744.1| Pseudomonas aeruginosa PA7, complete genome 40.9 0.002 1
dbj|AP009384.1| Azorhizobium caulinodans ORS 571 DNA, complet... 36.8 0.002 1
emb|AM420293.1| Saccharopolyspora erythraea NRRL2338 complete... 39.1 0.002 1
gb|CP001189.1| Gluconacetobacter diazotrophicus PAl 5, comple... 39.6 0.002 1
emb|AM406670.1| Azoarcus sp. BH72, complete genome 40.9 0.003 1
emb|AJ579650.1| Streptomyces tenebrarius tobramycin biosynthe... 40.0 0.003 1
emb|AL939122.1| Streptomyces coelicolor A3(2) complete genome... 34.5 0.003 1
gb|CP001683.1| Saccharomonospora viridis DSM 43017, complete ... 40.5 0.003 1
gb|AF521085.1| Streptomyces nanchangensis NS3226 polyether io... 37.7 0.003 1
emb|AM238663.1| Streptomyces ambofaciens ATCC 23877 left chro... 35.4 0.003 1
emb|CU914168.1| Ralstonia solanacearum strain IPO1609 Genome ... 39.6 0.003 1
emb|AJ875019.1| Streptoalloteichus hindustanus partial apramy... 35.0 0.003 1
Nous avons admis que non, mais avons nous raison?
De ce fait nous avons effectué des BLAST P environemental mais nous nous demandions s'il était pertinent d'effectuer un BLAST pour des séquences de 200 nucleotides.
LOGIN:NAME
|
CHerrmann09
23 Oct 2009 15:32
Maître de jeu |
Bonjour,
dans l'absolu, il est difficile de répondre à cette question sans regarder les alignements: on est en effet dans cette "zone grise" d'Evalue (twilight zone): l'Evalue est trop grand pour qu'on puisse conclure à l'homologie les yeux fermés, mais suffisamment petite pour que cette hypothèse ne puisse pas être exclu. Donc: regardez l'alignement pour décider!
Cela dit, je suis étonné du résultat: en prenant votre séquence d'ADN et en faisant un blastx contre nr, je trouve des résultats différents, qui semblent exclure une quelconque homologie:
Sequences producing significant alignments: (Bits) Value
ref|YP_226227.1| translation initiation factor IF-2 [Coryneba... 45.4 0.007 Gene info
ref|NP_601191.1| translation initiation factor IF-2 [Coryneba... 45.4 0.007 Gene info
ref|YP_001138710.1| translation initiation factor IF-2 [Coryn... 43.1 0.033 Gene info
gb|ABM21737.1| PdmP3 [Actinomadura hibisca] 42.7 0.044
ref|NP_827011.1| hypothetical protein SAV_5834 [Streptomyces ... 42.7 0.044 Gene info
ref|YP_290671.1| hypothetical protein Tfu_2615 [Thermobifida ... 41.6 0.097 Gene info
ref|ZP_05516893.1| hypothetical protein ShygA5_26437 [Strepto... 40.8 0.17
ref|YP_001378638.1| ribonuclease R [Anaeromyxobacter sp. Fw10... 40.0 0.28 Gene info
sp|P10163.3|PRB4_HUMAN RecName: Full=Basic salivary proline-r... 39.3 0.48 Gene info
gb|AAI30387.1| PRB4 protein [Homo sapiens] 39.3 0.48 Gene info
gb|EAW96231.1| proline-rich protein BstNI subfamily 4 [Homo s... 39.3 0.48 Gene info
ref|YP_716992.1| hypothetical protein FRAAL6865 [Frankia alni... 39.3 0.48 Gene info
gb|AAB50687.2| parotid 'o' protein; Po [Homo sapiens] 39.3 0.48 Gene info
ref|NP_420996.1| hypothetical protein CC_2193 [Caulobacter cr... 39.3 0.48 Gene info
emb|CAA30542.1| Po protein [Homo sapiens] 39.3 0.48 Gene info
ref|XP_002536116.1| conserved hypothetical protein [Ricinus c... 30.0 0.56 Gene info
ref|XP_002093095.1| GE21134 [Drosophila yakuba] >gb|EDW92807.... 38.5 0.82 Gene info
ref|ZP_01465528.1| fibrillar collagen chain FAp1 alpha [Stigm... 38.5 0.82
ref|XP_854250.1| PREDICTED: similar to PRKC, apoptosis, WT1, ... 38.5 0.82 UniGene infoGene info
ref|XP_422575.2| PREDICTED: similar to WDC146 [Gallus gallus] 38.1 1.1 Gene info
gb|AAR13814.1| major ampullate spidroin-2 [Argiope amoena] 38.1 1.1
gb|AAR13807.1| major ampullate spidroin-2 doubling 2 [synthet... 38.1 1.1
gb|AAR13808.1| major ampullate spidroin-2 [Argiope amoena] 38.1 1.1
gb|AAR13806.1| major ampullate spidroin-2 doubling 1 [synthet... 38.1 1.1
gb|AAR13810.1| major ampullate spidroin-2 [Argiope amoena] 38.1 1.1
ref|XP_002457454.1| hypothetical protein SORBIDRAFT_03g007560... 37.7 1.4 Gene info
ref|XP_002191591.1| PREDICTED: SWI/SNF related, matrix associ... 37.7 1.4 UniGene infoGene info
ref|ZP_03312093.1| hypothetical protein DESPIG_02018 [Desulfo... 37.7 1.4
gb|EAW96230.1| proline-rich protein BstNI subfamily 3 [Homo s... 37.7 1.4 Gene info
ref|XP_418841.2| PREDICTED: similar to DPY-19-like protein 1 ... 37.7 1.4 UniGene infoGene info
ref|NP_006240.4| proline-rich protein BstNI subfamily 3 precu... 37.7 1.4 UniGene infoGene info
gb|AAH96211.1| PRB3 protein [Homo sapiens] 37.7 1.4 Gene info
ref|YP_439859.1| polyketide synthase, putative [Burkholderia ... 37.7 1.4 Gene info
ref|ZP_05061403.1| secretion protein HlyD [gamma proteobacter... 37.4 1.8
ref|YP_001103592.1| PE-PGRS family protein [Saccharopolyspora... 37.4 1.8
Je ne comprends pas comment vous avez obtenu le résultat que vous donnez dans le message... |