Rabail 28 Apr 2011 14:34 Contribution non évaluée |
>ORF number 1 in reading frame 2 on the direct strand extends from base 293 to base 868. ATGCTTAATATAGATTTTCATGAAACTATTGGGCAAAGTCAATTTTCAGAAAAAGGAAAA CAAATAGTTATTAATGCATTAGAAAGACAAATAGCTAAAAGAGAAGATGATGGAGCTGTT TGTGTTTTATTAAATAGTAAGAAAAAGTATATTTTAAGAAGCAATCAAACAGCATCTTAT ATTACTCAGGATTTAGGAGCAGCAGTTGAAAGATTTAAAAAACATAATTTTAATAAAATG ATTTATATAACTGACTTTAGACAAAAACAGCATTTTGAAAGTTTATCTTTATTATTAAAA GCAATGAAATATGATTTTGCAGATAATCTTATACATTTACCTTTTGGAACTGTAAAATTT GGAAAAGAAATCATGGCTACAAGAAAAGGACAAATAATTCTTTTAAAAGATGTTTTAAAT AAAACAATTGAAAAAGCAAAAAACAATATAAAAAAGAGAAAAACAAAAGGAAGTCCTGAA AAAGTCGGAGTAGGAGCAATAAAATACATTATTTTAAAAAATGAACCTAAAAAAGATATT CAATTTTCATGGGAATCAGCATTATCTTTTGAAGGA
// The orf found over NCBI had a length of 822 bases whereas there was only one orf found over sequence manipulation suite with a length of 575 nucleotides in reading frame +2 with a start codon taken as ATG.The posted orf represents that of SMS orf finder. // we'd take our result as a true positive since it fulfills the limit of at least 150 codons.Total codons being 191. |