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Forum "ORF finding"

Thread subject: Known vs Novel

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Known vs Novel
Ccile
1 Mar 2017 0:44
Non evaluated contribution

Hi, 

 

How can we make the difference between Known and Novel ? I have the following ORF and I have 241 sequences in BLAST (with e-value < e-10) but I don't know if the fonction is known or not. Where can I find this information ? 

>Translation of ORF number 1 in reading frame 3 on the direct strand.
SRTMIRSFDAWEPVLRHTPASPDRNRSGDVTTAVEALATEGDMEVGVWEHSVGVSADVEV
EEVFLVLSGRGTVTCDSGGRIDLAPGVVGLLPMGAQTTWTVTEPLRKVWITLS*

The blast result (Expires on 03-02 05:59 am) on nr with max = 20000 and 1e-10 for the threshold.

https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Get&RID=BC06P067016

Thank you

Best regard

Ccile