Forum "Domaines conservés: INTERPRO"

Thread subject: Pas de Domaines protéiques !!

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Pas de Domaines protéiques !!
equipe1
10 Dec 2009 17:49
Contribution: Refer to Rule Book & FAQ
Bonjour,
Quand je lance ma séquence protéque (sens indirect, ORF 2) pour chercher des domaines protéiques contres les trois banques(PROSITE,INTERPRO & PFam) j'ai rien comme HITS.
ma question : est ce que je continues sur la meme piste et je commence a faire le Blast, ou bien je change ma séquence ? ,( je prond celle qui est sur le sens Directe ?)
merci.

RÉSULTATS BRUTS:

ORF Finder results
Results for 965 residue sequence "GOS_1075030 ADN génomique (Panama Canal: Lake Gatun)" starting "TCCTGTAATC"
>ORF number 1 in reading frame 1 on the direct strand extends from base 697 to base 912.
GAAAAACAATTAATGGCAACAGGCACAGTTAAGTGGTTCAACTCTGAAAAGGGCTTCGGC
TTCATTGCAGTAGATGGTGGCGGACAAGATGTATTCGTTCACTACTCAGCTATCCAAGGC
AATGGCTATAAGTCTCTTAACGAGGGTCAGTCAGTATCTTTCGAGGTCGTCCAGGGTCCA
AAGGGCCCACAGGCTGATCAAGTAACTGCTAACTAA

>Translation of ORF number 1 in reading frame 1 on the direct strand.
EKQLMATGTVKWFNSEKGFGFIAVDGGGQDVFVHYSAIQGNGYKSLNEGQSVSFEVVQGP
KGPQADQVTAN*

No ORFs were found in reading frame 2.

No ORFs were found in reading frame 3.

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ORF Finder results
Results for 965 residue sequence "GOS_1075030 ADN génomique (Panama Canal: Lake Gatun)" starting "TCCTGTAATC"
>ORF number 1 in reading frame 1 on the reverse strand extends from base 343 to base 582.
AAACATTCAAAAAAACTAACTTCGAGCGTCCGACTATGCGCAAGCCTACCCGCTTATAAG
GATGGGCTTTGCCACAAGCAAATCGGGAGTGGACAAAGCGCCCAATTAGAGTGGAAAATT
AGCTATCTGCTCTCAAAGCAAGTGCTTTTGGACTGGGGAAGGTGCAAATTGCGCCTTGCT
AGGAGCAAGATCAATGAAACGTGGATTTCTAACTATCCACTCATGCCCGCTGGCACTTAA


>Translation of ORF number 1 in reading frame 1 on the reverse strand.
KHSKKLTSSVRLCASLPAYKDGLCHKQIGSGQSAQLEWKISYLLSKQVLLDWGRCKLRLA
RSKINETWISNYPLMPAGT*

>ORF number 1 in reading frame 2 on the reverse strand extends from base 452 to base 964.
AGTGGAAAATTAGCTATCTGCTCTCAAAGCAAGTGCTTTTGGACTGGGGAAGGTGCAAAT
TGCGCCTTGCTAGGAGCAAGATCAATGAAACGTGGATTTCTAACTATCCACTCATGCCCG
CTGGCACTTAAAAGTCATGTCGAATGGGCGATCGAAAGCACTTTGGGTCCACTTCAGGTG
AATTGGCGAAACCAACCATTAGCGGCCGGAACTCAACGAACTCAGATTGAATGGCGTTCA
GATGAGAATTTAGCTTCGGTGCTTGCCAGTGCATTGAAGGGTTGGCATTACCTAAGATTT
GAAATCACCTTTGACTCTGATTTTTTTAGATTTACACCAGAACTTGGGTTACACCGTGCA
ACAATTGATCAACTTGGGTCCATTTTACTTACCGAGAATCAAATCAAATCCGCCCTGTCA
AAGACCGATGAAGTTATTCGTCAATCGCTAGATGTGGCGCTTGGCACAGAGTGGGAAATA
GCGCTAGAGCATTTCCGTGGGGTAGATTACAGG

>Translation of ORF number 1 in reading frame 2 on the reverse strand.
SGKLAICSQSKCFWTGEGANCALLGARSMKRGFLTIHSCPLALKSHVEWAIESTLGPLQV
NWRNQPLAAGTQRTQIEWRSDENLASVLASALKGWHYLRFEITFDSDFFRFTPELGLHRA
TIDQLGSILLTENQIKSALSKTDEVIRQSLDVALGTEWEIALEHFRGVDYR

No ORFs were found in reading frame 3.
ETalla09
12 Dec 2009 11:01
Game master
Bonjour,

Bien sur, vous continuez avec le Blast, etc.....