Forum "Analyse phylogénétique"

Sujet de discussion: enracinement d'arbre par taxa du groupe ext

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enracinement d'arbre par taxa du groupe ext
ivan-fay
2 Mar 2023 18:53
Contribution non évaluée

Je crois et j'espere toucher au but.

Est-il possible d'enraciner l'arbre comme cela?

 

 

NEWICK TREE MANIPULATION

                                                                   +--------------------------+ BPB1-E-value3e-84-Bacteria-Proteobacteria-Betaprot                                                      
                                                        +----------+ 0.88                                                                                                                               
                                                        |          +---------------------------------+ BPZ1-E-value7e-84-Bacteria-Proteobacteria-Zetaprot                                               
                                                        |                                                                                                                                               
                                               +--------+ 0.68                        +-------+ BPECCC1-E-value6e-82-Bacteria-Proteobacteria-Epsil                                                      
                                               |        |                    +--------+ 0.84                                                                                                            
                                               |        +--------------------+ 0.99   +----------+ BPGEEB1-E-value8e-83-Bacteria-Proteobacteria-Gamma                                                   
                                      +--------+ 0.88                        |                                                                                                                          
                                      |        |                             +----+ BPGEMP1-E-value3e-84-Bacteria-Proteobacteria-Gamma                                                                  
                                      |        |                                                                                                                                                        
                                      |        +---------------------+ BPG1-E-value6e-92-Bacteria-Proteobacteria-Gammapro                                                                               
                                      |                                                                                                                                                                 
                                      |                                                                                                | BPA1-E-value5e-164-Bacteria-Proteobacteria-Alphapr             
                                      |                                                                                      +---------+                                                                
                                      |                                                                                     ++ 0.99    | BPAP1-E-value3e-164-Bacteria-Proteobacteria-Alphap             
                                      |                                                                                     ||                                                                          
                                      |           +-------------------------------------------------------------------------++0.00-------+ OMRGCv2-11017080-Translation-168-1070-indirect-str           
                                      |           |                                                                         |                                                                           
                                      |           |                                                                         +-------------+ BPA2-E-value1e-146-Bacteria-Proteobacteria-Alphapr          
                                      |           |                                                                                                                                                     
                                      |           |                                                                 +---------+ BPAEEPE1-E-value3e-92-Bacteria-Proteobacteria-Alph                      
                                      |           |                                                     +-----------+ 0.98                                                                              
                                      |           |                                                     |           +----------+ BPAKKK2-E-value2e-92-Bacteria-Proteobacteria-Alpha                     
                                      |           |                                                +----+ 0.20                                                                                          
 +------------------------------------+ 0.30      |                                                |    |              +-----------+ BPAEEE1-E-value1e-95-Bacteria-Proteobacteria-Alpha                 
 |                                    |           |                                                |    +--------------+ 0.99                                                                           
 |                                    |           |                                      +---------+ 0.94              +--------+ BPAS1-E-value2e-93-Bacteria-Proteobacteria-Alphapr                    
 |                                    |           |                                      |         |                                                                                                    
 |                                    |           |                             +--------+ 0.87    +----------------------------------+ BPAKKK1-E-value5e-89-Bacteria-Proteobacteria-Alpha              
 |                                    |           |                             |        |                                                                                                              
 |                                    |           |                            ++ 0.55   +----------------------------------------------------------+ BPARG1-E-value9e-89-Bacteria-Proteobacteria-Alphap
 |                                    |           |                            ||                                                                                                                       
 |                                    |           |                            |+---------------------------+ BPARRR1-E-value1e-90-Bacteria-Proteobacteria-Alpha                                        
 |                                    |           |                            |                                                                                                                        
 |                                    |           |                            |              +------------------------+ BPARAAA1-E-value9e-86-Bacteria-Proteobacteria-Alph                             
 |                                    |           |                            |         +----+ 0.29                                                                                                    
 |                                    |           |                            |         |    +-------------------------+ BPASSO1-E-value6e-90-Bacteria-Proteobacteria-Alpha                            
 |                                    |           |                            |         |                                                                                                              
 |                                    |           |                            |    +----+ 0.85  +---------------------+ BPA5-E-value4e-92-Bacteria-Proteobacteria-Alphapro                             
 |                                    |           |                            |    |    |    +--+ 0.71                                                                                                 
 |                                    |           |                            |    |    |    |  +-----------------------------------+ BPAHRT1-E-value9e-90-Bacteria-Proteobacteria-Alpha               
 |                                    |           |                        +---+ 0.60    |    |                                                                                                         
 |                                    +-----------+ 1.00                   |   |    |    +----+ 0.80                 +------------------------+ BPA3-E-value7e-94-Bacteria-Proteobacteria-Alphapro      
 |                                                |                        |   |    |         |       +--------------+ 0.97                                                                             
 |                                                |                        |   |+---+ 0.38    +-------+ 0.84         +----------------+ BPARTN1-E-value3e-97-Bacteria-Proteobacteria-Alpha              
 |                                                |                        |   ||   |                 |                                                                                                 
 |                                                |                        |   ||   |                 +-------------------------+ BPARKAA1-E-value6e-101-Bacteria-Proteobacteria-Alp                    
 |                                                |                        |   ||   |                                                                                                                   
 |                                                |                        |   ||   |                                       +-----+ BPA4-E-value2e-93-Bacteria-Proteobacteria-Alphapro                  
 |                                                |                        |   ||   |            +--------------------------+ 1.00                                                                      
=|                                                |                        |   ||   +------------+ 0.97                     +------+ BPA6-E-value3e-91-Bacteria-Proteobacteria-Alphapro                 
 |                                                |                 +------+ 0.81 0.62           |                                                                                                      
 |                                                |                 |      |    |                +----------------------------+ BPARRM1-E-value9e-87-Bacteria-Proteobacteria-Alpha                      
 |                                                |                 |      |    |                                                                                                                       
 |                                                |                 |      |    |                                    +-----------------+ BPA8-E-value1e-87-Bacteria-Proteobacteria-Alphapro             
 |                                                |                 |      |    |                               +----+ 0.47                                                                             
 |                                                |                 |      |    |                    +----------+ 0.94----------+ BPASSS1-E-value3e-92-Bacteria-Proteobacteria-Alpha                    
 |                                                |                 |      |    |                    |          |                                                                                       
 |                                                |                 |      |    +--------------------+ 0.99     +----------------+ BPASS1-E-value8e-92-Bacteria-Proteobacteria-Alphap                   
 |                                                |                 |      |                         |                                                                                                  
 |                                                |                 |      |                         +-----------+ BPASSSS1-E-value1e-93-Bacteria-Proteobacteria-Alph                                   
 |                                                |        +--------+ 0.84 |                                                                                                                            
 |                                                |        |        |      +------------------------------------------------------------+ BPA7-E-value1e-89-Bacteria-Proteobacteria-Alphapro            
 |                                                |        |        |                                                                                                                                   
 |                                                |        |        |                                                   +-------+ BPARRR2-E-value6e-87-Bacteria-Proteobacteria-Alpha                    
 |                                                |        |        |                                             +-----+ 0.69                                                                          
 |                                                |        |        |                            +----------------+ 0.99+---------------+ BPARRT1-E-value8e-91-Bacteria-Proteobacteria-Alpha            
 |                                                +--------+ 0.90   |                            |                |                                                                                     
 |                                                         |        |        +-------------------+ 0.98           +-------------------------+ BPARPP1-E-value9e-87-Bacteria-Proteobacteria-Alpha        
 |                                                         |        |        |                   |                                                                                                      
 |                                                         |        +--------+ 0.92              +---------------+ BPAH1-E-value2e-91-Bacteria-Proteobacteria-Alphapr                                   
 |                                                         |                 |                                                                                                                          
 |                                                         |                 +-------------------------+ BPAHM1-E-value1e-87-Bacteria-Proteobacteria-Alphap                                             
 |                                                         |                                                                                                                                            
 |                                                         +----------------------------------------+ BPA9-E-value6e-86-Bacteria-Proteobacteria-Alphapro                                                
 |                                                                                                                                                                                                      
 +------------------------------------+ BPOSSS1-E-value2e-83-Bacteria-Proteobacteria-Oligo                                                                                                              
                                                                                                                                                                                                        
 |-------------------------------|------------------------------|-------------------------------|-------------------------------|--------------------                                                   
 0                            0.25                            0.5                            0.75                               1                                                                       
 substitutions/site     
Meglecz23CTES
2 Mar 2023 19:08
Maître de jeu

Non. La racine devrait être entre le groupe d’étude et le groupe extérieur.

ivan-fay
2 Mar 2023 23:16
Contribution non évaluée

Après avoir visionné la video je ne comprends pas pourquoi la technique ne fonctionne pas pour mon arbre

Je vais vous detailler ma démarche

 

 

Etape 1: Je lance mon arbre sur newick et obtiens

 

                                                                                        +------------------+ BPB1-E-value3e-84-Bacteria-Proteobacteria-Betaprot                     
                                                                                 +------+ 0.88                                                                                      
                                                                                 |      +-----------------------+ BPZ1-E-value7e-84-Bacteria-Proteobacteria-Zetaprot                
                                                                                 |                                                                                                  
                                                                          +------+ 0.68              +-----+ BPECCC1-E-value6e-82-Bacteria-Proteobacteria-Epsil                     
                                                                          |      |             +-----+ 0.84                                                                         
                                                                          |      +-------------+ 0.99+-------+ BPGEEB1-E-value8e-83-Bacteria-Proteobacteria-Gamma                   
                                                                    +-----+ 0.88               |                                                                                    
                                                                    |     |                    +--+ BPGEMP1-E-value3e-84-Bacteria-Proteobacteria-Gamma                              
                                                            +-------+ 0.30|                                                                                                         
                                                            |       |     +---------------+ BPG1-E-value6e-92-Bacteria-Proteobacteria-Gammapro                                      
                                                            |       |                                                                                                               
                                                            |       +--------------------------------------------------+ BPOSSS1-E-value2e-83-Bacteria-Proteobacteria-Oligo         
                                                            |                                                                                                                       
                                                            |                                             +------+ BPAEEPE1-E-value3e-92-Bacteria-Proteobacteria-Alph               
                                                            |                                    +--------+ 0.98                                                                    
                                                            |                                    |        +-------+ BPAKKK2-E-value2e-92-Bacteria-Proteobacteria-Alpha              
                                                            |                                 +--+ 0.20                                                                             
                                                            |                                 |  |          +-------+ BPAEEE1-E-value1e-95-Bacteria-Proteobacteria-Alpha            
                                                            |                                 |  +----------+ 0.99                                                                  
                                                            |                          +------+ 0.94        +-----+ BPAS1-E-value2e-93-Bacteria-Proteobacteria-Alphapr              
                                                            |                          |      |                                                                                     
                                                            |                    +-----+ 0.87 +-----------------------+ BPAKKK1-E-value5e-89-Bacteria-Proteobacteria-Alpha          
                                                            |                    |     |                                                                                            
                                                            |                   ++ 0.55+----------------------------------------+ BPARG1-E-value9e-89-Bacteria-Proteobacteria-Alphap
                                                            |                   ||                                                                                                  
                                                            |                   |+------------------+ BPARRR1-E-value1e-90-Bacteria-Proteobacteria-Alpha                            
                                                            |                   |                                                                                                   
                                                            |                   |          +----------------+ BPARAAA1-E-value9e-86-Bacteria-Proteobacteria-Alph                    
                                                            |                   |      +---+ 0.29                                                                                   
                                                            |                   |      |   +----------------+ BPASSO1-E-value6e-90-Bacteria-Proteobacteria-Alpha                    
                                                            |                   |      |                                                                                            
                                                            |                   |   +--+ 0.85+--------------+ BPA5-E-value4e-92-Bacteria-Proteobacteria-Alphapro                    
                                                            |                   |   |  |   +-+ 0.71                                                                                 
                                                            |                   |   |  |   | +------------------------+ BPAHRT1-E-value9e-90-Bacteria-Proteobacteria-Alpha          
        +---------------------------------------------------+ 1.00           +--+ 0.60 |   |                                                                                        
        |                                                   |                |  |   |  +---+ 0.80         +-----------------+ BPA3-E-value7e-94-Bacteria-Proteobacteria-Alphapro    
        |                                                   |                |  |   |      |    +---------+ 0.97                                                                    
        |                                                   |                |  |+--+ 0.38 +----+ 0.84    +-----------+ BPARTN1-E-value3e-97-Bacteria-Proteobacteria-Alpha          
        |                                                   |                |  ||  |           |                                                                                   
        |                                                   |                |  ||  |           +-----------------+ BPARKAA1-E-value6e-101-Bacteria-Proteobacteria-Alp              
        |                                                   |                |  ||  |                                                                                               
        |                                                   |                |  ||  |                          +---+ BPA4-E-value2e-93-Bacteria-Proteobacteria-Alphapro             
        |                                                   |                |  ||  |       +------------------+ 1.00                                                               
        |                                                   |                |  ||  +-------+ 0.97             +-----+ BPA6-E-value3e-91-Bacteria-Proteobacteria-Alphapro           
        |                                                   |            +---+ 0.810.62     |                                                                                       
        |                                                   |            |   |   |          +--------------------+ BPARRM1-E-value9e-87-Bacteria-Proteobacteria-Alpha               
        |                                                   |            |   |   |                                                                                                  
        |                                                   |            |   |   |                        +------------+ BPA8-E-value1e-87-Bacteria-Proteobacteria-Alphapro         
        |                                                   |            |   |   |                     +--+ 0.47                                                                    
        |                                                   |            |   |   |              +------+ 0.94-----+ BPASSS1-E-value3e-92-Bacteria-Proteobacteria-Alpha              
        |                                                   |            |   |   |              |      |                                                                            
        |                                                   |            |   |   +--------------+ 0.99 +-----------+ BPASS1-E-value8e-92-Bacteria-Proteobacteria-Alphap             
        | 0.00                                              |            |   |                  |                                                                                   
        |                                                   |            |   |                  +-------+ BPASSSS1-E-value1e-93-Bacteria-Proteobacteria-Alph                        
        |                                                   |     +------+ 0.84                                                                                                     
        |                                                   |     |      |   +------------------------------------------+ BPA7-E-value1e-89-Bacteria-Proteobacteria-Alphapro        
        |                                                   |     |      |                                                                                                          
        |                                                   |     |      |                                   +----+ BPARRR2-E-value6e-87-Bacteria-Proteobacteria-Alpha              
        |                                                   |     |      |                               +---+ 0.69                                                                 
        |                                                   |     |      |                  +------------+ 0.99--------+ BPARRT1-E-value8e-91-Bacteria-Proteobacteria-Alpha         
        |                                                   +-----+ 0.90 |                  |            |                                                                          
 +------+ 0.99                                                    |      |     +------------+ 0.98       +-----------------+ BPARPP1-E-value9e-87-Bacteria-Proteobacteria-Alpha     
 |      |                                                         |      |     |            |                                                                                       
 |      |                                                         |      +-----+ 0.92       +-----------+ BPAH1-E-value2e-91-Bacteria-Proteobacteria-Alphapr                        
 |      |                                                         |            |                                                                                                    
 |      |                                                         |            +-----------------+ BPAHM1-E-value1e-87-Bacteria-Proteobacteria-Alphap                               
 |      |                                                         |                                                                                                                 
 |      |                                                         +----------------------------+ BPA9-E-value6e-86-Bacteria-Proteobacteria-Alphapro                                 
=|      |                                                                                                                                                                           
 |      |---------+ BPA2-E-value1e-146-Bacteria-Proteobacteria-Alphapr                                                                                                              
 |      |                                                                                                                                                                           
 |      +-------+ OMRGCv2-11017080-Translation-168-1070-indirect-str                                                                                                                
 |                                                                                                                                                                                  
 | BPA1-E-value5e-164-Bacteria-Proteobacteria-Alphapr                                                                                                                               
 |                                                                                                                                                                                  
 | BPAP1-E-value3e-164-Bacteria-Proteobacteria-Alphap                                                                                                                               
                                                                                                                                                                                    
 |---------------------|---------------------|---------------------|---------------------|---------------------|-----------------                                                   
 0                  0.25                   0.5                  0.75                     1                  1.25                                                                    
 substitutions/site

 

 

 

 

 

Etape 1: J'enracine mon arbre sur un taxa du groupe extérieur pour obtenir un arbre similaire à la video ici 

 BPG1-E-value6e-92-Bacteria-Proteobacteria-Gammapro  (surligné en jaune). J'obtiens alors:

 

 

 

                                                                                                                   | BPA1-E-value5e-164-Bacteria-Proteobacteria-Alphapr             
                                                                                                         +---------+                                                                
                                                                                                        ++ 0.99    | BPAP1-E-value3e-164-Bacteria-Proteobacteria-Alphap             
                                                                                                        ||                                                                          
                                +-----------------------------------------------------------------------++0.00-------+ OMRGCv2-11017080-Translation-168-1070-indirect-str           
                                |                                                                       |                                                                           
                                |                                                                       +-------------+ BPA2-E-value1e-146-Bacteria-Proteobacteria-Alphapr          
                                |                                                                                                                                                   
                                |                                                                +---------+ BPAEEPE1-E-value3e-92-Bacteria-Proteobacteria-Alph                     
                                |                                                    +-----------+ 0.98                                                                             
                                |                                                    |           +----------+ BPAKKK2-E-value2e-92-Bacteria-Proteobacteria-Alpha                    
                                |                                               +----+ 0.20                                                                                         
                                |                                               |    |              +-----------+ BPAEEE1-E-value1e-95-Bacteria-Proteobacteria-Alpha                
                                |                                               |    +--------------+ 0.99                                                                          
                                |                                     +---------+ 0.94              +--------+ BPAS1-E-value2e-93-Bacteria-Proteobacteria-Alphapr                   
                                |                                     |         |                                                                                                   
                                |                             +-------+ 0.87    +---------------------------------+ BPAKKK1-E-value5e-89-Bacteria-Proteobacteria-Alpha              
                                |                             |       |                                                                                                             
                                |                           +-+ 0.55  +---------------------------------------------------------+ BPARG1-E-value9e-89-Bacteria-Proteobacteria-Alphap
                                |                           | |                                                                                                                     
                                |                           | +--------------------------+ BPARRR1-E-value1e-90-Bacteria-Proteobacteria-Alpha                                       
                                |                           |                                                                                                                       
                                |                           |               +-----------------------+ BPARAAA1-E-value9e-86-Bacteria-Proteobacteria-Alph                            
                                |                           |          +----+ 0.29                                                                                                  
                                |                           |          |    +-----------------------+ BPASSO1-E-value6e-90-Bacteria-Proteobacteria-Alpha                            
                                |                           |          |                                                                                                            
                                |                           |    +-----+ 0.85 +---------------------+ BPA5-E-value4e-92-Bacteria-Proteobacteria-Alphapro                            
                                |                           |    |     |   +--+ 0.71                                                                                                
                                |                           |    |     |   |  +----------------------------------+ BPAHRT1-E-value9e-90-Bacteria-Proteobacteria-Alpha               
                                |                        +--+ 0.60     |   |                                                                                                        
                     +----------+ 1.00                   |  |    |     +---+ 0.80                +------------------------+ BPA3-E-value7e-94-Bacteria-Proteobacteria-Alphapro      
                     |          |                        |  |    |         |       +-------------+ 0.97                                                                             
                     |          |                        |  | +--+ 0.38    +-------+ 0.84        +----------------+ BPARTN1-E-value3e-97-Bacteria-Proteobacteria-Alpha              
                     |          |                        |  | |  |                 |                                                                                                
                     |          |                        |  | |  |                 +-------------------------+ BPARKAA1-E-value6e-101-Bacteria-Proteobacteria-Alp                   
                     |          |                        |  | |  |                                                                                                                  
                     |          |                        |  | |  |                                       +----+ BPA4-E-value2e-93-Bacteria-Proteobacteria-Alphapro                  
                     |          |                        |  | |  |            +--------------------------+ 1.00                                                                     
                     |          |                        |  | |  +------------+ 0.97                     +------+ BPA6-E-value3e-91-Bacteria-Proteobacteria-Alphapro                
                     |          |                 +------+ 0.81 0.62          |                                                                                                     
                     |          |                 |      |    |               +---------------------------+ BPARRM1-E-value9e-87-Bacteria-Proteobacteria-Alpha                      
                     |          |                 |      |    |                                                                                                                     
                     |          |                 |      |    |                                  +-----------------+ BPA8-E-value1e-87-Bacteria-Proteobacteria-Alphapro             
                     |          |                 |      |    |                              +---+ 0.47                                                                             
                     |          |                 |      |    |                   +----------+ 0.94----------+ BPASSS1-E-value3e-92-Bacteria-Proteobacteria-Alpha                   
                     |          |                 |      |    |                   |          |                                                                                      
                     |          |                 |      |    +-------------------+ 0.99     +---------------+ BPASS1-E-value8e-92-Bacteria-Proteobacteria-Alphap                   
            +--------+ 0.30     |                 |      |                        |                                                                                                 
            |        |          |                 |      |                        +-----------+ BPASSSS1-E-value1e-93-Bacteria-Proteobacteria-Alph                                  
            |        |          |        +--------+ 0.84 |                                                                                                                          
            |        |          |        |        |      +-----------------------------------------------------------+ BPA7-E-value1e-89-Bacteria-Proteobacteria-Alphapro           
            |        |          |        |        |                                                                                                                                 
            |        |          |        |        |                                                  +------+ BPARRR2-E-value6e-87-Bacteria-Proteobacteria-Alpha                    
            |        |          |        |        |                                            +-----+ 0.69                                                                         
            |        |          |        |        |                           +----------------+ 0.99+--------------+ BPARRT1-E-value8e-91-Bacteria-Proteobacteria-Alpha            
            |        |          +--------+ 0.90   |                           |                |                                                                                    
            |        |                   |        |       +-------------------+ 0.98           +-------------------------+ BPARPP1-E-value9e-87-Bacteria-Proteobacteria-Alpha       
            |        |                   |        |       |                   |                                                                                                     
 +----------+ 0.88   |                   |        +-------+ 0.92              +---------------+ BPAH1-E-value2e-91-Bacteria-Proteobacteria-Alphapr                                  
 |          |        |                   |                |                                                                                                                         
 |          |        |                   |                +-------------------------+ BPAHM1-E-value1e-87-Bacteria-Proteobacteria-Alphap                                            
 |          |        |                   |                                                                                                                                          
 |          |        |                   +---------------------------------------+ BPA9-E-value6e-86-Bacteria-Proteobacteria-Alphapro                                               
 |          |        |                                                                                                                                                              
 |          |        +-----------------------------------------------------------------------+ BPOSSS1-E-value2e-83-Bacteria-Proteobacteria-Oligo                                   
 |          |                                                                                                                                                                       
 |          |                  +--------------------------+ BPB1-E-value3e-84-Bacteria-Proteobacteria-Betaprot                                                                      
=|          |        +---------+ 0.88                                                                                                                                               
 |          |        |         +---------------------------------+ BPZ1-E-value7e-84-Bacteria-Proteobacteria-Zetaprot                                                               
 |          |        |                                                                                                                                                              
 |          +--------+ 0.68                      +--------+ BPECCC1-E-value6e-82-Bacteria-Proteobacteria-Epsil                                                                      
 |                   |                   +-------+ 0.84                                                                                                                             
 |                   +-------------------+ 0.99  +----------+ BPGEEB1-E-value8e-83-Bacteria-Proteobacteria-Gamma                                                                    
 |                                       |                                                                                                                                          
 |                                       +---+ BPGEMP1-E-value3e-84-Bacteria-Proteobacteria-Gamma                                                                                   
 |                                                                                                                                                                                  
 +----------+ BPG1-E-value6e-92-Bacteria-Proteobacteria-Gammapro                                                                                                                    
                                                                                                                                                                                    
 |------------------------------|------------------------------|------------------------------|------------------------------|---                                                   
 0                           0.25                            0.5                           0.75                              1                                                      
 substitutions/site                                                                                                                     

 

 

 

 

 

 

sur cet arbre nous distingons trés bien les alpha du groupe exterieur( alpha en orange) Il me suffit normalement d'enraciner en prennant un groupe du haut et un du bas. admettons les deux en jaune.
 

 

voila l'arbre que j'obtiens:

 

                                                                                     +-------------------+ BPB1-E-value3e-84-Bacteria-Proteobacteria-Betaprot                       
                                                                              +------+ 0.88                                                                                         
                                                                              |      +-------------------------+ BPZ1-E-value7e-84-Bacteria-Proteobacteria-Zetaprot                 
                                                                              |                                                                                                     
                                                                       +------+ 0.68               +------+ BPECCC1-E-value6e-82-Bacteria-Proteobacteria-Epsil                      
                                                                       |      |              +-----+ 0.84                                                                           
                                                                       |      +--------------+ 0.99+--------+ BPGEEB1-E-value8e-83-Bacteria-Proteobacteria-Gamma                    
                                                                +------+ 0.88                |                                                                                      
                                                                |      |                     +--+ BPGEMP1-E-value3e-84-Bacteria-Proteobacteria-Gamma                                
                                                        +-------+ 0.30 |                                                                                                            
                                                        |       |      +---------------+ BPG1-E-value6e-92-Bacteria-Proteobacteria-Gammapro                                         
                                                        |       |                                                                                                                   
                                                        |       +------------------------------------------------------+ BPOSSS1-E-value2e-83-Bacteria-Proteobacteria-Oligo         
                                                        |                                                                                                                           
                                                        |                                                +------+ BPAEEPE1-E-value3e-92-Bacteria-Proteobacteria-Alph                
                                                        |                                      +---------+ 0.98                                                                     
                                                        |                                      |         +-------+ BPAKKK2-E-value2e-92-Bacteria-Proteobacteria-Alpha               
                                                        |                                   +--+ 0.20                                                                               
                                                        |                                   |  |           +--------+ BPAEEE1-E-value1e-95-Bacteria-Proteobacteria-Alpha            
                                                        |                                   |  +-----------+ 0.99                                                                   
                                                        |                            +------+ 0.94         +-----+ BPAS1-E-value2e-93-Bacteria-Proteobacteria-Alphapr               
                                                        |                            |      |                                                                                       
                                                        |                     +------+ 0.87 +------------------------+ BPAKKK1-E-value5e-89-Bacteria-Proteobacteria-Alpha           
                                                        |                     |      |                                                                                              
                                                        |                    ++ 0.55 +------------------------------------------+ BPARG1-E-value9e-89-Bacteria-Proteobacteria-Alphap
                                                        |                    ||                                                                                                     
                                                        |                    |+--------------------+ BPARRR1-E-value1e-90-Bacteria-Proteobacteria-Alpha                             
                                                        |                    |                                                                                                      
                                                        |                    |           +-----------------+ BPARAAA1-E-value9e-86-Bacteria-Proteobacteria-Alph                     
                                                        |                    |       +---+ 0.29                                                                                     
                                                        |                    |       |   +-----------------+ BPASSO1-E-value6e-90-Bacteria-Proteobacteria-Alpha                     
                                                        |                    |       |                                                                                              
                                                        |                    |   +---+ 0.85+---------------+ BPA5-E-value4e-92-Bacteria-Proteobacteria-Alphapro                     
                                                        |                    |   |   |  +--+ 0.71                                                                                   
                                                        |                    |   |   |  |  +-------------------------+ BPAHRT1-E-value9e-90-Bacteria-Proteobacteria-Alpha           
 +------------------------------------------------------+ 1.00            +--+ 0.60  |  |                                                                                           
 |                                                      |                 |  |   |   +--+ 0.80           +-----------------+ BPA3-E-value7e-94-Bacteria-Proteobacteria-Alphapro     
 |                                                      |                 |  |   |      |     +----------+ 0.97                                                                     
 |                                                      |                 |  |+--+ 0.38 +-----+ 0.84     +------------+ BPARTN1-E-value3e-97-Bacteria-Proteobacteria-Alpha          
 |                                                      |                 |  ||  |            |                                                                                     
 |                                                      |                 |  ||  |            +------------------+ BPARKAA1-E-value6e-101-Bacteria-Proteobacteria-Alp               
 |                                                      |                 |  ||  |                                                                                                  
 |                                                      |                 |  ||  |                            +----+ BPA4-E-value2e-93-Bacteria-Proteobacteria-Alphapro             
 |                                                      |                 |  ||  |        +-------------------+ 1.00                                                                
 |                                                      |                 |  ||  +--------+ 0.97              +-----+ BPA6-E-value3e-91-Bacteria-Proteobacteria-Alphapro            
 |                                                      |             +---+ 0.810.62      |                                                                                         
 |                                                      |             |   |   |           +---------------------+ BPARRM1-E-value9e-87-Bacteria-Proteobacteria-Alpha                
 |                                                      |             |   |   |                                                                                                     
 |                                                      |             |   |   |                          +------------+ BPA8-E-value1e-87-Bacteria-Proteobacteria-Alphapro          
 |                                                      |             |   |   |                       +--+ 0.47                                                                     
 |                                                      |             |   |   |               +-------+ 0.94-----+ BPASSS1-E-value3e-92-Bacteria-Proteobacteria-Alpha               
 |                                                      |             |   |   |               |       |                                                                             
 |                                                      |             |   |   +---------------+ 0.99  +-----------+ BPASS1-E-value8e-92-Bacteria-Proteobacteria-Alphap              
 | 0.00                                                 |             |   |                   |                                                                                     
 |                                                      |             |   |                   +--------+ BPASSSS1-E-value1e-93-Bacteria-Proteobacteria-Alph                         
 |                                                      |      +------+ 0.84                                                                                                        
 |                                                      |      |      |   +---------------------------------------------+ BPA7-E-value1e-89-Bacteria-Proteobacteria-Alphapro        
 |                                                      |      |      |                                                                                                             
 |                                                      |      |      |                                     +----+ BPARRR2-E-value6e-87-Bacteria-Proteobacteria-Alpha               
 |                                                      |      |      |                                +----+ 0.69                                                                  
 |                                                      |      |      |                   +------------+ 0.99----------+ BPARRT1-E-value8e-91-Bacteria-Proteobacteria-Alpha         
 |                                                      +------+ 0.90 |                   |            |                                                                            
 |                                                             |      |     +-------------+ 0.98       +------------------+ BPARPP1-E-value9e-87-Bacteria-Proteobacteria-Alpha      
 |                                                             |      |     |             |                                                                                         
=|                                                             |      +-----+ 0.92        +-----------+ BPAH1-E-value2e-91-Bacteria-Proteobacteria-Alphapr                          
 |                                                             |            |                                                                                                       
 |                                                             |            +------------------+ BPAHM1-E-value1e-87-Bacteria-Proteobacteria-Alphap                                 
 |                                                             |                                                                                                                    
 |                                                             +-----------------------------+ BPA9-E-value6e-86-Bacteria-Proteobacteria-Alphapro                                   
 |                                                                                                                                                                                  
 |----------+ BPA2-E-value1e-146-Bacteria-Proteobacteria-Alphapr                                                                                                                    
 |                                                                                                                                                                                  
 |       | BPA1-E-value5e-164-Bacteria-Proteobacteria-Alphapr                                                                                                                       
 |-------+                                                                                                                                                                          
 | 0.99  | BPAP1-E-value3e-164-Bacteria-Proteobacteria-Alphap                                                                                                                       
 |                                                                                                                                                                                  
 +--------+ OMRGCv2-11017080-Translation-168-1070-indirect-str                                                                                                                      
                                                                                                                                                                                    
 |----------------------|-----------------------|----------------------|----------------------|----------------------|-----------                                                   
 0                   0.25                     0.5                   0.75                      1                   1.25                                                              
 substitutions/site                                                                                                                                                                 
               

 

 

 

Je ne comprends pas pourquoi je n'arrive pas a enraciner mon arbre correctement 

 

Meglecz23CTES
3 Mar 2023 10:24
Maître de jeu

Bonjour,


Merci pour vos explications précises. Cela m’aide à comprendre votre erreur.


Si l’arbre n’est pas enraciné correctement, alors la racine sépare en deux soit le groupe d’étude, soit le groupe extérieur, tandis que l’outre groupe est bien délimité par un nœud.


Par exemple, dans l’arbre de l’étape 1 (Etape 1: Je lance mon arbre sur newick et obtiens), le groupe extérieur est clairement délimité par un nœud avec la robustesse 0.3. Tous les descendants de ce nœud sont les non-alpha, et tous les non-alpha descendent de ce nœud.

Le groupe d’étude est coupé en deux par la racine (deux séquences sont un côté de la racine, les autres sont sur l’autre).

Il faut donc focaliser sur le groupe extérieur dans votre cas, car c’est le groupe qui est bien regroupé sous un nœud.

Il faut indiquer à l’outil de la visualisation de l’arbre de l’annotathon ce noeud, pour qu’il place la racine entre ce nœud (groupe extérieur) et toutes les autres séquences (groupe d’étude).


Pour le faire, il faut un identifiant de chacune des deux branches qui descend de ce nœud. Autrement dit, il faut sélectionner deux séquences dont l’ancêtre le plus récent se trouve à ce nœud.


Votre erreur était de choisir une séquence de groupe d’étude et une autre de groupe extérieur ce qui place nécessairement mal la racine.

 

Bon travail,
Emese Meglecz

ivan-fay
3 Mar 2023 12:16
Contribution non évaluée

CA FONCTIONNE !!!!!